To improve artificial insemination protocols in ovine species it is crucial to optimize sperm
quality evaluation after preservation technologies. Emerging technologies based on novel
biomolecules and related to redox balance and proteins involved in sperm motility such as
ProAKAP4 could be successfully applied in ram sperm evaluation. In this work, a
multiparametric analysis of fresh, cooled, and cryopreserved ram sperm was performed at
different complexity levels. Samples were evaluated in terms of motility (total motility …