Profiling of sugar nucleotides

M Rejzek, L Hill, ES Hems, S Kuhaudomlarp… - Methods in …, 2017 - Elsevier
… Herein we survey technical developments in sugar nucleotide profiling, with an emphasis …
approaches to sugar nucleotide profiling and on experience in our group. Profiling methods …

High-throughput profiling of nucleotides and nucleotide sugars to evaluate their impact on antibody N-glycosylation

TK Villiger, RF Steinhoff, M Ivarsson, T Solacroup… - Journal of …, 2016 - Elsevier
profiling of intracellular nucleotides and nucleotide sugars of … altered the intracellular
nucleotides and nucleotide sugars that are … altered the glycan profiles without affecting …

Profiling Cell Wall Monosaccharides and NucleotideSugars from Plants

C Rautengarten, JL Heazlewood… - Current protocols in …, 2019 - Wiley Online Library
… a complete profile of all major sugars in the … nucleotide sugar profile from plant extracts.
Here we describe detailed methodology to quantify nucleotide sugars within the cell and profile

Quantification of nucleotides and their sugar conjugates in biological samples: purposes, instruments and applications

X Qin, X Wang - Journal of Pharmaceutical and Biomedical Analysis, 2018 - Elsevier
Nucleotides and nucleotide sugars are one kind of the endogenous molecules that arouse
scientists’ attention. Nucleotides are basic units building DNA … Profiling of sugar nucleotides

Sugar nucleotide quantification using multiple reaction monitoring liquid chromatography/tandem mass spectrometry

AD Garcia, JL Chavez, Y Mechref - Rapid Communications in …, 2013 - Wiley Online Library
… , were established, the method was applied for the determination of sugar nucleotide profiles
of three breast cancer cells of differing phenotypes. The cell lines employed for this study …

Impact of nucleotide sugar metabolism on protein N-glycosylation in Chinese Hamster Ovary (CHO) cell culture

HM Naik, NI Majewska, MJ Betenbaugh - Current opinion in chemical …, 2018 - Elsevier
… Alternatively, high throughput methods not requiring chromatographic separation, such
MALDI-TOF MS, have been used to profile intracellular sugar nucleotides to study the impact of …

Rapid analysis of nucleotide-activated sugars by high-performance liquid chromatography coupled with diode-array detection, electrospray ionization mass …

M Ramm, JL Wolfender, EF Queiroz… - … of Chromatography A, 2004 - Elsevier
… for HPLC analysis of sugar nucleotides was established. … 11 of the most important sugar
nucleotides and compatibility with on-… Nucleotide co-factors of enzymatic reactions such as ATP …

An optimized method for extraction and quantification of nucleotides and nucleotide sugars from mammalian cells

IJ del Val, S Kyriakopoulos, KM Polizzi… - Analytical biochemistry, 2013 - Elsevier
… This article describes an optimized method for nucleotide and nucleotide sugar extraction
… to define the most suitable protocol for nucleotide and nucleotide sugar (NS) recovery. The …

Sugar activation for production of nucleotide sugars as substrates for glycosyltransferases in plants

LA Kleczkowski, D Decker - Journal of Applied Glycoscience, 2015 - jstage.jst.go.jp
… NDP-sugars (or, in one case, NMP-sugar), … nucleotide-sugar pyrophosphorylases (all
using NTP as one of the substrates and differing in sugar specificity) as well as nucleotide-sugar

An investigation of intracellular glycosylation activities in CHO cells: effects of nucleotide sugar precursor feeding

NSC Wong, L Wati, PM Nissom… - Biotechnology and …, 2010 - Wiley Online Library
… by profiling of nucleotide and nucleotide sugars over time … a CE method to profile 12
nucleotides and 7 nucleotide sugars. … with various combinations of nucleotide sugar precursors, to …