[HTML][HTML] Proliferation kinetics and differentiation potential of ex vivo expanded human bone marrow stromal cells: Implications for their use in cell therapy

A Banfi, A Muraglia, B Dozin, M Mastrogiacomo… - Experimental …, 2000 - Elsevier
OBJECTIVE: Bone marrow stromal cells (BMSC) are an attractive target for novel strategies
in the gene/cell therapy of hematologic and skeletal pathologies, involving BMSC in vitro
expansion/transfection and reinfusion. We investigated the effects of in vitro expansion on
BMSC pluripotentiality, proliferative ability, and bone-forming efficiency in vivo. MATERIALS
AND METHODS: BMSC from three marrow donors were cultured to determine their growth
kinetics. At each passage, their differentiation potential was verified by culture in inductive …
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