… promoters, similar 3-HP-inducible gene expression systems … as a carbon substrate nor its
biological degradation has been … that the expressions of these enzymes are induced by 3-HP. …

… Due to recent concerns over climate change and limited fossil reserves, the biological …
hybrid promoter with a dual regulatory system requires both IPTG and 3-HP for induction. To …

… on the biological production of fuels and chemicals from … and that the expression of the
degrading enzymes was induced by 3… The possibility that the hbdH4 promoter is induced by other …

… system in response to 3-HP and show that it is also induced … the PpmmsA nor PphpdH
promoter can be induced by the … HP-inducible system that can be utilised for synthetic biology and …

… expression of DhaS, a putative ald from Bacillus subtilis, which showed higher specificity
toward 3-hydroxypropionic acid … Among the promoters tested (tac and lac), the IPTG-induced …

… Microbial metabolic pathways for 3-HP biological production … However, Snf1 is also
responsible for inducing genes involved … To increase MCR activity, a dual-promoter approach was …

… Biologically, 3-HP can be produced from glucose or glycerol 1 . With glucose … method as
described previously 25 . The data were normalized against the un-induced mRNA expression …

… the use of synthetic biology methods (eg CRISPR toolbox, … repetitive tac promoters rather
than a single tac promoter to … By altering induction condition and medium components, the …

… -1 vector with the T7 promoter was adopted. The pct and … The cells were harvested after 6
h induction and centrifuged at … As propionate itself is a biological product and its biosynthesis …

… Despite the importance of 3-HP, its biological production … -1b with T7 promoter was used to
have a high expression. The … 0.45 ± 0.05 OD 600 to induce the expression of dhaB and aldH. …