Bats live in niches close to human surroundings. Pathogenic viruses, bacteria and fungi may be transferred from bats to humans. The purpose of these studies were the isolation and characterization of bacteria present in bat faeces. The examinations were conducted on faeces collected from the following bat species: Myotis myotis, Pipistrellus nathusii, Eptesicus serotinus and Vespertilio murinus. Some species of bat intestinal bacteria, such as: Enterobacter sp. and Hafnia alvei were identified. Gram-negative rods dominated in bat faeces. The amounts of bacterial cells were in the range of 0.5×10⁴-5×10⁶ g faeces. The antibiotic resistance analysis revealed that 23% of staphylococci were methicillin resistant. MRSA and methicillin-resistant S. sciuri strains were identified. Numerous bacteria isolated from bat faeces gave evidence of proteolytic and haemolytic activities.