The present research work was to assess the antidiabetic activity of petroleum ether, ethanolic and aqueous fruit extracts of Luffa cylindrica. Antidiabetic activity on alloxan induced hyperglycemic albino rats was carried out. The antihyperglycemic effect was determined by the estimation of fasting blood glucose and biochemical markers like serum urea, serum triglycerides and serum cholesterol. Glibenclamide was used as a reference standard drug. A marked rise in fasting blood glucose level was observed in diabetic control compare to normal control rats. Petroleum ether, ethanolic and aqueous fruit extracts of Luffa cylindrica significantly lowered the elevated serum blood glucose level on 7th day post treatment. Glibenclamide produced a significant reduction in blood glucose as compare to diabetic control. However, the effect of test extracts on fasting blood glucose level was found to be less potent than the reference standard drug, glibenclamide. Serum cholesterol, triglycerides and urea levels were decreased significantly by ethanolic fruit extract of Luffa cylindrica followed by petroleum ether and aqueous extracts on 7th day of post treatment, when compared to diabetic control group. However, the effect of these extracts on above said biochemical parameters was found to be less potent than the standard drug glibenclamide.