[HTML][HTML] Protein trans-Splicing and Cyclization by a Naturally Split Intein from the dnaE Gene ofSynechocystis Species PCC6803
TC Evans, D Martin, R Kolly, D Panne, L Sun… - Journal of Biological …, 2000 - ASBMB
A naturally occurring split intein from thednaE gene of Synechocystis sp. PCC6803 (Ssp
DnaE intein) has been shown to mediate efficientin vivo and in vitro trans-splicing in a
foreign protein context. A cis-splicing SspDnaE intein construct displayed splicing activity
similar to thetrans-splicing form, which suggests that the N-and C-terminal intein fragments
have a high affinity interaction. Anin vitro trans-splicing system was developed that used a
bacterially expressed N-terminal fragment of the Ssp DnaE intein and either a bacterially …
DnaE intein) has been shown to mediate efficientin vivo and in vitro trans-splicing in a
foreign protein context. A cis-splicing SspDnaE intein construct displayed splicing activity
similar to thetrans-splicing form, which suggests that the N-and C-terminal intein fragments
have a high affinity interaction. Anin vitro trans-splicing system was developed that used a
bacterially expressed N-terminal fragment of the Ssp DnaE intein and either a bacterially …