Withania somnifera L. member of family Solanaceae abundantly found in sub-tropical regions of the world, it is a multipurpose medicinal plant. Phytochemical screening of Withania somniferra L. was carried out to know about the medicinal potential of same plant. A qualitative analysis was performed to detect various bioactive compounds. The stem, leaves and fruits were individually subjected to phytochemical analysis, revealing the presence of carbohydrates, alkaloids, saponins, flavonoids. proteins, Anthocyanin, Quinones and Tanins which enable it for the developing of new drugs.