Objective The aim of this study was to quantify porcine vocal fold medial surface geometry
and three-dimensional geometric distortion induced by freezing the larynx, especially in the
region of the vocal folds. Study Design The medial surface geometries of five excised
porcine larynges were quantified and reported. Methods Five porcine larynges were imaged
in a micro-CT scanner, frozen, and rescanned. Segmentations and three-dimensional
reconstructions were used to quantify and characterize geometric features. Comparisons …

Quantifying the internal spatial distribution of the various tissue layers within the vocal folds
is important for voice production research. However, various researchers have shown that
freezing3, resection4, fixing in formalin5, and staining all have an effect to at least some
degree on tissue geometry, making it difficult to obtain reliable geometrical data. Tayama et
al. 3 measured canine vocal fold geometry in the mid-membranous coronal plane at various
stages of thawing after quick freezing. They showed that the frozen tissue expands slightly …