RACE-based amplification of cDNA and expression of a luciferin-regenerating enzyme (LRE): An attempt towards persistent bioluminescent signal
In the firefly light organ, luciferin-regenerating enzyme (LRE) plays an important role in the
recycling of oxyluciferin into luciferin. In this study, the LRE cDNA was cloned from total RNA
extracted from lantern of Lampyris turkestanicus (an Iranian firefly) using PCR-based
techniques including reverse transcription-polymerase chain reaction, 5′-RACE (5′-rapid
amplification of cDNA ends) and 3′-RACE. The full-length cDNA encoding LRE of L.
turkestanicus (T-LRE) was 1047 with 924bp open-reading frame encoding the protein of 307 …
recycling of oxyluciferin into luciferin. In this study, the LRE cDNA was cloned from total RNA
extracted from lantern of Lampyris turkestanicus (an Iranian firefly) using PCR-based
techniques including reverse transcription-polymerase chain reaction, 5′-RACE (5′-rapid
amplification of cDNA ends) and 3′-RACE. The full-length cDNA encoding LRE of L.
turkestanicus (T-LRE) was 1047 with 924bp open-reading frame encoding the protein of 307 …
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