… substantial length because natural polymerases incorporate modified nucleotides inefficiently.
… functional T7 RNA polymerase variants based on the ability of a T7 RNA polymerase to …

… RNA coronavirus responsible for the pandemic of the Severe Acute Respiratory Syndrome
(COVID-19). RNA … , as SARS-CoV-2 RNA dependent RNA polymerase (RdRp). Mutation rate …

… RNA polymerase, we have generated a panel of six highly active and orthogonal polymerase:…
The high activity of T7 RNA polymerase and its derivatives can potentially lead to toxicity, …

… of T7 RNA polymerase (RNAP) at a set of promoter variants having all possible single base
pair (bp) substitutions. The polymerase … Promoter variants that would require initiation at the …

… G/L variant that has all but replaced the initial D/P variant. Our … RNA-dependent RNA
polymerase, is a necessary alteration that led to the epidemiological success of the present variant …

… Consequently, the activity and processivity barrier required for a polymerase variant to
self-replicate and ”survive” a round of selection is lowered. Therefore, spCSR should be a …

… recognition by all three nuclear RNA polymerases (pol I, II, and Ill). … pol I, II, and Ill all require
this factor. A mutation of TBP has been identified that will function with pol I but not with pol II …

… h, Reconstituted Pfu RNA polymerase variants form PICs according to band shift assays.
The following point mutations were introduced in the B-linker-binding surface on the clamp …

… In this study, Harrison et al. isolated two viral RNA dependent RNA polymerase (RdRp) mutants
… for resistance to RNA mutagens with the goal of identifying higher fidelity RdRp variants. …

… characterize the genomic binding profiles of six H1 variants. Most H1s bind to specific sites,
… on the elongation of RNA polymerase II (RNAPII). Thus, H1 variants determine splicing fate …