… from the ERF transcription factor (Hiratsu et al., 2003). We measured the transcriptional
regulation conferred by these chimeric dCas9-based transcriptional regulators using gRNA …

… for both RNA-guided genome editing and gene regulation in a … and independently targeted
gene regulation and editing in … We provide a basal set of orthogonal RNA-guided proteins …

… and gene regulation can be highly specific for the target gene locus in cell culture systems 15
. To explore potential off-target gene regulation effects with RNA-guided … RNA-guided gene …

… were associated with gene activation. Based … gene activation could be a novel regulatory
mechanism for the small RNA-Ago pathway and named it as RNA-induced transcriptional gene …

… (41) Moreover, RNA editing can impact alternative splicing patterns and translation, adding
an additional layer of complexity to post-transcriptional regulation. The dynamic nature of …

… -p65AD can effectively activate reporter gene expression, suggesting that dCas9 can serve
as a generic and modular platform for different types of transcriptional control, including both …

… Small RNA-guided gene silencing in mouse oocytes is mainly executed by retrotransposons
and pseudogene-derived endogenous small interfering RNAs (endo-siRNAs), rather than …

… To repurpose the Cas9/CRISPR for genome regulation instead of genome editing, here we
… as a platform for RNA-guided transcription regulation. The transcription of arbitrary genes …

… Gene expression is strictly controlled in many biol-ogical processes, such as development
and diseases. Transcription factors regulate gene … , and modulate transcription through their …

… genomes that direct chromatin modifications and gene expression, synthetic biologists
have repurposed prokaryotic RNA-guided genome defense systems such as CRISPR to edit …