Recombinant protein expression at low temperatures under the transcriptional control of the major Escherichia coli cold shock promoter cspA
JA Vasina, F Baneyx - Applied and environmental microbiology, 1996 - Am Soc Microbiol
JA Vasina, F Baneyx
Applied and environmental microbiology, 1996•Am Soc MicrobiolA transcriptional gene fusion between the cspA promoter and the lacZ gene was constructed
to assess the usefulness of cold shock promoters for low-temperature protein expression.
Synthesis of beta-galactosidase was efficiently repressed at 37 degrees C but rapidly
induced upon transfer to the 15-to-30 degrees C range, leading to a three-to fivefold
increase in specific activity relative to control cultures. Although the initial rates of beta-
galactosidase accumulation at 20 degrees C were twice those measured at 15 degrees C …
to assess the usefulness of cold shock promoters for low-temperature protein expression.
Synthesis of beta-galactosidase was efficiently repressed at 37 degrees C but rapidly
induced upon transfer to the 15-to-30 degrees C range, leading to a three-to fivefold
increase in specific activity relative to control cultures. Although the initial rates of beta-
galactosidase accumulation at 20 degrees C were twice those measured at 15 degrees C …
A transcriptional gene fusion between the cspA promoter and the lacZ gene was constructed to assess the usefulness of cold shock promoters for low-temperature protein expression. Synthesis of beta-galactosidase was efficiently repressed at 37 degrees C but rapidly induced upon transfer to the 15-to-30 degrees C range, leading to a three- to fivefold increase in specific activity relative to control cultures. Although the initial rates of beta-galactosidase accumulation at 20 degrees C were twice those measured at 15 degrees C, prolonged incubation at 20 degrees C, but not 15 degrees C, led to a dilution of activity due to repression of the promoter and cell division.
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