Recommended mass spectrometry-based strategies to identify ricin-containing samples

SR Kalb, DM Schieltz, F Becher, C Astot… - Toxins, 2015 - mdpi.com
… -based methods to detect, differentiate and quantify ricin and RCA120 in nine blinded samples
… Overall, MS-based assays successfully identified all samples containing ricin or RCA120 …

Unambiguous identification of ricin and abrin with advanced mass spectrometric assays

SR Kalb, F Becher - … in Forensic Proteomics: Protein Identification …, 2019 - ACS Publications
identify ricin or abrin and to detect RIP activity in samples with … The best unique ricin
peptides with favorable physico-… methods and affinity enrichment are reviewed in this paper. …

Constructing a tandem mass spectral library for forensic ricin identification

I O'Bryon, AE Tucker, BLD Kaiser… - Journal of proteome …, 2019 - ACS Publications
… Of the set of consensus PSMs for a given peptide ion, the one with the best database search
… and methods to actual forensic samples is paramount. To that end, we employed a series of …

Ricin-like proteins from the castor plant do not influence liquid chromatography-mass spectrometry detection of ricin in forensically relevant samples

ED Merkley, SC Jenson, JS Arce, AM Melville… - Toxicon, 2017 - Elsevier
identification developed by the Organization for the Prohibition of Chemical Weapons (OPCW)
recommend … met by mass spectrometry-based untargeted proteomics methods. Additional …

Rapid, sensitive and reliable ricin identification in serum samples using LC–MS/MS

L Feldberg, E Elhanany, O Laskar, O Schuster - Toxins, 2021 - mdpi.com
… , rapid and simple analytical methods for unambiguous ricin identification from clinical …
To the best of our knowledge, the identification of ricin so late post exposure had not been …

Mass spectrometric detection and differentiation of enzymatically active abrin and ricin combined with a novel affinity enrichment technique

KK Drinkard, JR Barr, SR Kalb - Chemical research in toxicology, 2024 - ACS Publications
… Several strategies have been adopted to detect ricin or abrin, … 40 μL of these beads was
found to yield the best results with … we determined it was best for toxin extraction from samples. …

Rapid detection of ricin at trace levels in complex matrices by asialofetuin-coated beads and bottom-up proteomics using high-resolution mass spectrometry

P Piquet, J Saadi, F Fenaille, SR Kalb… - Analytical and …, 2024 - Springer
… to human or food samples. Mass spectrometrybased methods provide specific toxin … by
the signal of the 3 best ricin peptides usually monitored by mass spectrometry, normalized to the …

[PDF][PDF] Protein identification and characterization through peptide mass spectrometry

T Bergström - Acta Universitatis Agriculturae Sueciae, 2016 - core.ac.uk
… A large part of this thesis deals with development of sample preparation methods, so no
further … A set of peptides unique to ricin has been identified, and it has been suggested that the …

… as an Internal Retention Time Standard for Increased Confidence in Forensic Identification of Ricin by Untargeted Nanoflow Liquid Chromatography-Tandem Mass …

ED Merkley, KE Burnum-Johnson… - Analytical …, 2019 - ACS Publications
… Get article recommendations from ACS based on references … Qualitative methods to detect
ricin are therefore needed. … LC for ricin peptides in castor samples, we have analyzed a set of …

Probabilistic limit of detection for ricin identification using a shotgun proteomics assay

NC Heller, AM Garrett, ED Merkley… - Analytical …, 2019 - ACS Publications
… Our criteria are based on the recommendations of several … Other OPCW recommendations
concerned with detecting the … -20) and two alternative sample cleanup methods (strong cation …