Redox regulation of cAMP-dependent protein kinase signaling: kinase versus phosphatase inactivation

KM Humphries, JK Pennypacker, SS Taylor - Journal of biological chemistry, 2007 - ASBMB
Many components of cellular signaling pathways are sensitive to regulation by oxidation and
reduction. Previously, we described the inactivation of cAMP-dependent protein kinase
(PKA) by direct oxidation of a reactive cysteine in the activation loop of the kinase. In the
present study, we demonstrate that in HeLa cells PKA activity follows a biphasic response to
thiol oxidation. Under mild oxidizing conditions, or short exposure to oxidants, forskolin-
stimulated PKA activity is enhanced. This enhancement was blocked by sulfhydryl reducing …
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