Repurposing the Endogenous Type IE CRISPR/Cas System for Gene Repression in Gluconobacter oxydans WSH-003
Z Qin, Y Yang, S Yu, L Liu, Y Chen, J Chen… - ACS synthetic …, 2021 - ACS Publications
Gluconobacter oxydans is well-known for its incomplete oxidizing capacity and has been
widely applied in industrial production. However, genetic tools in G. oxydans are still scarce
compared with model microorganisms, limiting its metabolic engineering. This study aimed
to develop a clustered regularly interspaced short palindromic repeats interference
(CRISPRi) system based on the typical type IE endogenous CRISPR/CRISPR-associated
proteins (Cas) system in G. oxydans WSH-003. The nuclease Cas3 in this system was …
widely applied in industrial production. However, genetic tools in G. oxydans are still scarce
compared with model microorganisms, limiting its metabolic engineering. This study aimed
to develop a clustered regularly interspaced short palindromic repeats interference
(CRISPRi) system based on the typical type IE endogenous CRISPR/CRISPR-associated
proteins (Cas) system in G. oxydans WSH-003. The nuclease Cas3 in this system was …
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