… Response of antibiotic resistance genes (ARGs) and heavy metal resistance genes (HMRGs)
to two different temperature sequences (ie, mesophilic-thermophilic and thermophilic-…

… the response of heavy metal resistant microorganisms (HMRMs) and antibiotic resistant …
In recent years, the development of metagenomic sequencing has provided an effective …

… were analyzed by quantitative polymerase chain reaction (… Annealing temperature and
expected amplicon sizes of each … The sequence of ARGs occurrence was in the order of tet, sul …

… The primer sequences and annealing temperatures for the seven resistance genes … in each
treatment changed over time in response to different concentrations of Cd and SD. The main …

… High-throughput quantitative polymerase chain reaction (qPCR) coupled with amplicon
sequencing and metal … In our study, the high temperature phase during the composting was likely …

… temperature. After a desired duration, the reactors were cooled in 0 C water to terminate
reaction. … MGEs, typically including integrons, transposons, insertion sequences and plasmids …

… as promoters to antibiotic resistance gene (ARG) … a set of sequencing batch reactors (SBRs)
and micro-scale microfluidic chips were established to quantify the impacts of heavy metals (…

… of pile temperature on the fate of antibiotic resistance genes (… subtypes responding differently
to composting temperature … to classify the sequences into different taxonomic groups. The …

… in thermostatic water bath shakers to obtain suitable temperature (25 ± 1 C) and were …
and sequenced. The raw sequences obtained were deposited in Sequence Read Archive (…

… in a multiple reaction monitoring (MRM) mode. A Zorbax XDB C18 column (2.1 mm × 150 mm,
3.5 μm) with a column temperature of 40 C was used to separate the antibiotics … sequence …