Membrane contact sites (MCSs) link organelles to coordinate cellular functions across space
and time. Although viruses remodel organelles for their replication cycles, MCSs remain
largely unexplored during infections. Here, we design a targeted proteomics platform for
measuring MCS proteins at all organelles simultaneously and define functional virus-driven
MCS alterations by the ancient beta-herpesvirus human cytomegalovirus (HCMV).
Integration with super-resolution microscopy and comparisons to herpes simplex virus (HSV …