Robust and versatile arrayed libraries for human genome-wide CRISPR activation, deletion and silencing
JA Yin, L Frick, MC Scheidmann, T Liu, C Trevisan… - BioRxiv, 2022 - biorxiv.org
… Arrayed CRISPR libraries extend the scope of gene-perturbation screens but require large
… genome-wide arrayed libraries for human gene ablation (19,936 plasmids), activation, and …
… genome-wide arrayed libraries for human gene ablation (19,936 plasmids), activation, and …
Arrayed CRISPR libraries for the genome-wide activation, deletion and silencing of human protein-coding genes
JA Yin, L Frick, MC Scheidmann, T Liu… - Nature Biomedical …, 2024 - nature.com
… of highly active, versatile CRISPR arrayed libraries that overcome the limitations of existing
… underscore the general robustness of arrayed CRISPR activation screens with the T.gonfio …
… underscore the general robustness of arrayed CRISPR activation screens with the T.gonfio …
Enhancing the genome editing toolbox: genome wide CRISPR arrayed libraries
E Metzakopian, A Strong, V Iyer, A Hodgkins… - Scientific reports, 2017 - nature.com
… locus, the resulting RNA (crRNA) and trans-activating RNA (… resulting in error-prone repair
with deletions or insertions (INDELs). In … Individual sgRNAs were cloned into a highly versatile …
with deletions or insertions (INDELs). In … Individual sgRNAs were cloned into a highly versatile …
Multi-functional genome-wide CRISPR system for high throughput genotype–phenotype mapping
… pooled or arrayed guide RNA (gRNA) libraries that can … both libraries, interactions between
the deletion and activation targets … After incubation at 30 C for 16 h with vigorous shaking, the …
the deletion and activation targets … After incubation at 30 C for 16 h with vigorous shaking, the …
[PDF][PDF] Robust and Versatile Arrayed Libraries for Human Genome-Wide CRISPR Activation, Deletion and Silencing
… a new generation of highly active, robust, generic and versatile CRISPR arrayed libraries …
our arrayed libraries with a novel algorithm to select the optimal combination of four sgRNAs (…
our arrayed libraries with a novel algorithm to select the optimal combination of four sgRNAs (…
Generation of Two Human Genome-Wide CRISPR Arrayed Libraries and Study of Thrombopoietin Receptor MPL in Prion Toxicity
Y Wu - 2024 - zora.uzh.ch
… system when studying the essential genes whose deletion caused a lethal effect. CRISPRi
… a robust and versatile multiplexed gRNA arrayed library for both CRISPR activation and …
… a robust and versatile multiplexed gRNA arrayed library for both CRISPR activation and …
Genome-wide CRISPR screen reveals v-ATPase as a drug target to lower levels of ALS protein ataxin-2
… We developed a fluorescence-activated cell sorting (FACS)-based screening method using
pooled CRISPR-Cas9-mediated genome-wide deletion libraries in conjunction with antibody …
pooled CRISPR-Cas9-mediated genome-wide deletion libraries in conjunction with antibody …
Genome-wide CRISPR screens in primary human T cells reveal key regulators of immune function
… cancer treatments, achieving durable responses in a subset … of T cell suppression and
activation, but systematic strategies … >75,000 guides in the genome-wide (GW) library. Bottom: LFC …
activation, but systematic strategies … >75,000 guides in the genome-wide (GW) library. Bottom: LFC …
[HTML][HTML] High-content CRISPR screening
… In arrayed CRISPR screens, different perturbations are … CRISPR gRNA libraries may be
genome-wide or focused on … CRISPRko introduces small insertions and deletions that typically …
genome-wide or focused on … CRISPRko introduces small insertions and deletions that typically …
Functional genomics via CRISPR–Cas
… Deletions and chromosomal rearrangements induced by … Arrayed library screening often
requires specialized … Genome-wide Cas9 screens are increasingly popular due to their …
requires specialized … Genome-wide Cas9 screens are increasingly popular due to their …