The role of PKC and RhoA/ROCK pathways in the phasic activities in the rectal smooth
muscles (RSM) in the basal state is not known. We examined this issue by determining the
effects of PKC inhibitors (calphostin C and Gö-6850) and a ROCK inhibitor (Y-27632) on the
slow-rate (∼ 3/min) and fast-rate (∼ 25/min) phasic activities. We also examined the
corresponding signal transduction cascades and the PKC and ROCK enzymatic activities in
the RSM in the basal state. PKC inhibition with calphostin C and Gö-6850 (10− 5 M) caused …

41 Background & Aims: Role of PKC and RhoA/ROCK pathways in the phasic activities in
the 42 rectal smooth muscles (RSM) in the basal state is not known. Methods: We examined
this issue 43 by determining the effects of PKC inhibitors (calphostin C and Gö6850) and
ROCK inhibitor 44 (Y27632) on the slow (~ 3/min) and fast rate (~ 25/min) rate phasic
activities. We also examined 45 the corresponding signal transduction cascades, and the
PKC and ROCK enzymatic activities in 46 the RSM, in the basal state. Results: PKC …