Salicylic acid (SA) is a plant hormone that plays an important role in induction of plant defense against a variety of biotic and abiotic stresses through morphological, physiological and biochemical mechanisms. In our study we have observed that plants grown in presence of SA show healthy appearance (Fusarium wilt symptoms) and an optimal vegetative growth compared to inoculated plants. The aimed objective of this work is to deduce if an exogenous application of salicylic acid can induce the accumulation of soluble phenolic compound in leaves of hydroponic tomato plants. This result is confirmed 24h after treatment when a gradual increase in peroxidase activity 24h after SA roots feeding were observed in roots and leaves. RT-PCR expression analysis of the gene encoding phenyl-amenia-lyase (PAL) and peroxidase (PR9) confirmed those results. The present work also showed that the PR-proteins such as glucanase (PR4), and chitinase (PR2) quickly responded to salicylic treatment and a high accumulation level of PR4 and PR2 transcripts was detected earlier at 48h and 72h post treatment through root feeding. These results suggest that SA at 200µM is safe to tomato plants and could be recommended for the stimulation of Fusarium defense which could be used biologic management programs.