Scaling-up techniques for the nanofabrication of cell culture substrates via two-photon polymerization for industrial-scale expansion of stem cells
Stem-cell-based therapies require a high number (106–109) of cells, therefore in vitro
expansion is needed because of the initially low amount of stem cells obtainable from
human tissues. Standard protocols for stem cell expansion are currently based on
chemically-defined culture media and animal-derived feeder-cell layers, which expose cells
to additives and to xenogeneic compounds, resulting in potential issues when used in
clinics. The two-photon laser polymerization technique enables three-dimensional micro …
expansion is needed because of the initially low amount of stem cells obtainable from
human tissues. Standard protocols for stem cell expansion are currently based on
chemically-defined culture media and animal-derived feeder-cell layers, which expose cells
to additives and to xenogeneic compounds, resulting in potential issues when used in
clinics. The two-photon laser polymerization technique enables three-dimensional micro …
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