Introduction Due to a lack of spatial-temporal resolution at the single cell level, the etiologies
of the bone dysfunction caused by diseases such as normal aging, osteoporosis, and the
metabolic bone disease associated with chronic kidney disease (CKD) remain largely
unknown. Methods To this end, flow cytometry and scRNAseq were performed on long bone
cells from Sost-cre/Ai9+ mice, and pure osteolineage transcriptomes were identified,
including novel osteocyte-specific gene sets. Results Clustering analysis isolated osteoblast …

Methods: To this end, flow cytometry and scRNAseq were performed on long bone cells from
Sost-cre/Ai9+ mice, and pure osteolineage transcriptomes were identified, including novel
osteocyte-specific gene sets. Results: Clustering analysis isolated osteoblast precursors that
expressed Tnc, Mmp13, and Spp1, and a mature osteoblast population defined by Smpd3,
Col1a1, and Col11a1. Osteocytes were demarcated by Cd109, Ptprz1, Ramp1, Bambi,
Adamts14, Spns2, Bmp2, WasI, and Phex. We validated our in vivo scRNAseq using …