… for TrwC-mediated site-specific integration. We have obtained TrwC-mediated site-specific
integration on minimal oriTw and human target sequences and into the Escherichia coli …

… for TrwC-mediated site-specific integration. We have obtained TrwC-mediated site-specific
integration on minimal oriTw and human target sequences and into the Escherichia coli …

… , based on in vivo TrwC-DNA transfer to mammalian cells by bacterial conjugation (45). The
… mediate site-specific integration of the incoming foreign DNA into specific human targets. …

… were cotransfected into the human cells (Fig. 2). We determined the DNA sequence of this
PCR … as expected for integrase-mediated site-specific recombination between attP and attB. …

… for TrwC-mediated site-specific integration. We have obtained TrwC-mediated site-specific
integration on minimal oriTw and human target sequences and into the Escherichia coli …

… human cell, although the efficiency of site-specific integration … of the transferred DNA into
DNA sequences of human origin (… role as a site-specific integrase in human cells, which would …

… Bacterial protein TrwC catalyzes integration into human sequences and can be delivered to
human … The use of SSRs (see Glossary), which directly integrate foreign DNA into a specific …

… Inserting custom designed DNA sequences into the … integration of foreign DNA to rapidly
prototype synthetic gene networks in mammalian cells. (88) In this study, site-specific integration …

… attP site into endogenous sequences in the human genome. … into the human genome, either
by random integration or at a … to catalyze precise site-specific integration into inserted wild-…

… these cells covalently attached to a site-specific integrase with potential target sequences in
… Site-specific integration of foreign DNA into minimal bacterial and human target sequences …