Small and large scale genomic DNA isolation protocol for chickpea (Cicer arietinum L.), suitable for molecular marker and transgenic analyses
Chickpea is an important food legume crop with high nutritional value. Lack of appropriate
DNA isolation protocol is a limiting factor for any molecular studies of this crop. The present
report describes a rapid and efficient protocol for small and large scale preparation of
superior quality and quantity of DNA from four cultivars (JG62, WR315, C235 and
ICCV89314) compared to that of earlier reports. The yield of DNA through both the methods
was estimated to be approximately 80 μg per g of plant tissue. Both small and large scale …
DNA isolation protocol is a limiting factor for any molecular studies of this crop. The present
report describes a rapid and efficient protocol for small and large scale preparation of
superior quality and quantity of DNA from four cultivars (JG62, WR315, C235 and
ICCV89314) compared to that of earlier reports. The yield of DNA through both the methods
was estimated to be approximately 80 μg per g of plant tissue. Both small and large scale …
Small and large scale genomic DNA isolation protocol for chickpea (Cicer arietinum L.), suitable for molecular marker and transgenic analyses.
DC Dipankar Chakraborti, AS Anindya Sarkar… - 2006 - cabidigitallibrary.org
Chickpea is an important food legume crop with high nutritional value. Lack of appropriate
DNA isolation protocol is a limiting factor for any molecular studies of this crop. The present
report describes a rapid and efficient protocol for small and large scale preparation of
superior quality and quantity of DNA from four cultivars (JG62, WR315, C235 and
ICCV89314) compared to that of earlier reports. The yield of DNA through both the methods
was estimated to be approximately 80 µg per g of plant tissue. Both small and large scale …
DNA isolation protocol is a limiting factor for any molecular studies of this crop. The present
report describes a rapid and efficient protocol for small and large scale preparation of
superior quality and quantity of DNA from four cultivars (JG62, WR315, C235 and
ICCV89314) compared to that of earlier reports. The yield of DNA through both the methods
was estimated to be approximately 80 µg per g of plant tissue. Both small and large scale …
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