Spot pattern of leopard Danio is caused by mutation in the zebrafish connexin41.8 gene
M Watanabe, M Iwashita, M Ishii, Y Kurachi… - EMBO …, 2006 - embopress.org
EMBO reports, 2006•embopress.org
Leopard, a well‐known zebrafish mutant that has a spotted skin pattern instead of stripes, is
a model for the study of pigment patterning. To understand the mechanisms underlying
stripe formation, as well as the spot variation observed in leopard, we sought to identify the
gene responsible for this phenotype. Using positional cloning, we identified the leopard
gene as an orthologue of the mammalian connexin 40 gene. A variety of different leopard
alleles, such as leo t1, leo tq270 and leo tw28, show different skin‐pattern phenotypes. In …
a model for the study of pigment patterning. To understand the mechanisms underlying
stripe formation, as well as the spot variation observed in leopard, we sought to identify the
gene responsible for this phenotype. Using positional cloning, we identified the leopard
gene as an orthologue of the mammalian connexin 40 gene. A variety of different leopard
alleles, such as leo t1, leo tq270 and leo tw28, show different skin‐pattern phenotypes. In …
Leopard, a well‐known zebrafish mutant that has a spotted skin pattern instead of stripes, is a model for the study of pigment patterning. To understand the mechanisms underlying stripe formation, as well as the spot variation observed in leopard, we sought to identify the gene responsible for this phenotype. Using positional cloning, we identified the leopard gene as an orthologue of the mammalian connexin 40 gene. A variety of different leopard alleles, such as leot1, leotq270 and leotw28, show different skin‐pattern phenotypes. In this manuscript we show that the mutation in allele leot1 is a nonsense mutation, whereas alleles leotq270 and leotw28 contain the missense mutations I202F and I31F, respectively. Patch‐clamp experiments of connexin hemichannels demonstrated that the I202F substitution in allele leotq270 disrupted the channel function of connexin41.8. These results demonstrate that mutations in this gene lead to a variety of leopard spot patterns.
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