[HTML][HTML] Src homologous and collagen (Shc) protein binds to F-actin and translocates to the cytoskeleton upon nerve growth factor stimulation in PC12 cells
D Thomas, SD Patterson, RA Bradshaw - Journal of Biological Chemistry, 1995 - ASBMB
Immunoprecipitates of metabolically labeled PC12 cells consistently contained a 43-kDa
protein that was associated with Shc, a signal-transducing protein with a single SH2 domain.
Following affinity chromatography with immobilized recombinant glutathione S-transferase
(GST)-Shc fusion protein, the 43-kDa protein was identified as actin by mass spectrometry
and immunoblotting. Cosedimentation experiments using purified actin and GST-Shc
showed that Shc binds directly to F-actin, confirming Shc-actin interaction in vivo. Various …
protein that was associated with Shc, a signal-transducing protein with a single SH2 domain.
Following affinity chromatography with immobilized recombinant glutathione S-transferase
(GST)-Shc fusion protein, the 43-kDa protein was identified as actin by mass spectrometry
and immunoblotting. Cosedimentation experiments using purified actin and GST-Shc
showed that Shc binds directly to F-actin, confirming Shc-actin interaction in vivo. Various …
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