Although perilipin 2 (Plin2) has been shown to bind lipids with high affinity, the Plin2 lipid
binding site has yet to be defined. This is of interest since Plin2's affinity for lipids has been
suggested to be important for lipid droplet biogenesis and intracellular triacylglycerol
accumulation. To define these regions, mouse Plin2 and several deletion mutants
expressed as recombinant proteins and in mammalian cells were assessed by molecular
modeling, fluorescence binding, circular dichroic, and fluorescence resonance energy …