This article focuses on the trace element content of fish feed and shrimp sampled from aquaculture ponds in Bangladesh which were determined using a Flame Atomic Absorption Spectrometer (FAAS), Cold Vapour-Atomic Absorption Spectrometer (CV-AAS) and the Hydride Generation- Atomic Absorption Spectrometer (HG-AAS). Analytical methods combining closed digestion, followed by determination with HG-AAS, CV-AAS and FAAS was developed for study of Arsenic, Cadmium, Lead and Mercury concentration at very low levels in fish feed. The method has detection limits of 0.1 µg/g, 0.1 µg/g, 0.1 µg/g, and 0.03 µg/g for As, Cd, Pb and Hg respectively. Oyster tissue and lobster hepatopancreas (TORT-2) reference standards were used in trace element recovery and method validations. The range of elemental concentrations (mg/kg diet, dry wt.) of fish feed were: As 0.14-0.91, Cd <0.1-2.1, Pb <0.1-8.57 and Hg was found to be below the detection limit (<0.03). The results show that the elemental concentrations (mg/kg wet wt.) of arsenic, cadmium, mercury, lead and chromium in shrimp from aquaculture sources were below the detection limit and were low compared with maximum limits for contaminants in shrimp set by the EU.