Supercritical carbon dioxide extraction efficiency for carotenes from carrots by RSM

PJ Vega, MO Balaban, CA Sims… - Journal of Food …, 1996 - Wiley Online Library
PJ Vega, MO Balaban, CA Sims, SF O'keefe, JA Cornell
Journal of Food Science, 1996Wiley Online Library
Increasing demand for β‐carotene has resulted in a growing interest in its extraction from
natural sources. Carotenes were extracted from carrot pulp (press cake) using SC‐CO2.
Three levels of pressure (20.7, 27.6, and 34.5 MPa), temperature (40, 55, and 70° C), and
ethanol co‐solvent (0, 5, 10% wt) were studied. Percentages α‐, β‐and total carotenes
extracted were determined by HPLC and spectrophotometric methods. A maximum of 99.5%
of β‐carotene was extracted using 10% ethanol. Concentration of ethanol and temperature …
Abstract
Increasing demand for β‐carotene has resulted in a growing interest in its extraction from natural sources. Carotenes were extracted from carrot pulp (press cake) using SC‐CO2. Three levels of pressure (20.7, 27.6, and 34.5 MPa), temperature (40, 55, and 70°C), and ethanol co‐solvent (0, 5, 10% wt) were studied. Percentages α‐, β‐ and total carotenes extracted were determined by HPLC and spectrophotometric methods. A maximum of 99.5% of β‐carotene was extracted using 10% ethanol. Concentration of ethanol and temperature were the most important factors in increasing extraction yield. A response surface model was developed from the results: %β‐carotene extraction = 8.4558 + 0.8816 T– 10.7650 E + 0.1843 T*E + 1.9019 E2– 0.0261 E2*T, where T = temperature (°C), E = ethanol (%). R2= 0.973.
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