Membrane protein variants with diminished conformational stability often exhibit enhanced
cellular expression at reduced growth temperatures. The expression of" temperature-
sensitive" variants is also typically sensitive to corrector molecules that bind and stabilize the
native conformation. There are many examples of temperature-sensitive rhodopsin variants,
the misfolding of which is associated with the molecular basis of retinitis pigmentosa. In this
work, we employ deep mutational scanning to compare the effects of reduced growth …