Abstract Background TILLING (T argeting I nduced L ocal L esions in G enomes) is a reverse
genetic technique based on the use of a mismatch-specific enzyme that identifies mutations
in a target gene through heteroduplex analysis. We tested this technique in Caenorhabditis
elegans, a model organism in which genomics tools have been well developed, but
limitations in reverse genetics have restricted the number of heritable mutations that have
been identified. Results To determine whether TILLING represents an effective reverse …

Background: TILLING (Targeting Induced Local Lesions in Genomes) is a reverse genetic
technique based on the use of a mismatch-specific enzyme that identifies mutations in a
target gene through heteroduplex analysis. We tested this technique in Caenorhabditis
elegans, a model organism in which genomics tools have been well developed, but
limitations in reverse genetics have restricted the number of heritable mutations that have
been identified. Results: To determine whether TILLING represents an effective reverse …