We previously described that cell-wide cytosolic Ca 2+ transients evoked by inositol
trisphosphate (IP 3) are generated by two modes of Ca 2+ liberation from the
ER;'punctate'release via an initial flurry of transient Ca 2+ puffs from local clusters of IP 3
receptors, succeeded by a spatially and temporally 'diffuse'Ca 2+ liberation. Those findings
were derived using statistical fluctuation analysis to monitor puff activity which is otherwise
masked as global Ca 2+ levels rise. Here, we devised imaging approaches to resolve …

We previously described that cell-wide cytosolic Ca2+ transients evoked by inositol
trisphosphate (IP3) are generated by two modes of Ca2+ liberation from the
ER;'punctate'release via an initial flurry of transient Ca2+ puffs from local clusters of IP3
receptors, succeeded by a spatially and temporally'diffuse'Ca2+ liberation. Those findings
were derived using statistical fluctuation analysis to monitor puff activity which is otherwise
masked as global Ca2+ levels rise. Here, we devised imaging approaches to resolve …