Tetraploid Complementation Assay

M Gertsenstein - Advanced Protocols for Animal Transgenesis: An ISTT …, 2011 - Springer
… Aggregation of tetraploidcomplemented by tetraploid embryos they colonize the embryo
proper, the amnion, the allantois as well as the mesoderm layer of the yolk sac, while tetraploid

iPS cells produce viable mice through tetraploid complementation

X Zhao, W Li, Z Lv, L Liu, M Tong, T Hai, J Hao, C Guo… - nature, 2009 - nature.com
… produced by the most stringent tetraploid complementation assay, indicative of a failure to
… viable, fertile live-born progeny by tetraploid complementation. These iPS cells maintain a …

Production of mice using iPS cells and tetraploid complementation

XY Zhao, Z Lv, W Li, F Zeng, Q Zhou - Nature Protocols, 2010 - nature.com
… animals through the tetraploid complementation assay. Our … cells tested by tetraploid
complementation assay, compared … iPS animal through tetraploid complementation with only one-…

Mouse embryos' fusion for the tetraploid complementation assay

M Gertsenstein - Cell Fusion: Overviews and Methods, 2015 - Springer
Tetraploid embryos are generated by the fusion of two blastomeres of the mouse embryo.
The applications of tetraploid complementation assay and … Tetraploid complementation assay

Tetraploid complementation proves pluripotency of induced pluripotent stem cells derived from adipose tissue

C Zhou, X Cai, Y Fu, X Wei, N Fu, J Xie… - Cell Proliferation, 2015 - Wiley Online Library
… Recently, pluripotency of induced pluripotent stem (iPS) cells has been displayed after
producing adult mice, in tetraploid complementation assays. These studies lead us to the last …

Viable mice produced from three-factor induced pluripotent stem (iPS) cells through tetraploid complementation

L Kang, T Wu, Y Tao, Y Yuan, J He, Y Zhang, T Luo… - Cell research, 2011 - nature.com
… differentiation into teratomas and tetraploid blastocyst complementation assay were performed.
Tetraploid complementation assay was further performed to examine whether the three-…

Rapid generation of gene-targeted EPS-derived mouse models through tetraploid complementation

H Li, C Zhao, J Xu, Y Xu, C Cheng, Y Liu, T Wang… - Protein & …, 2019 - academic.oup.com
… (E) Bar chart shows the proportion of full-term, breathing, adult mice derived from transferred
embryos in the tetraploid complementation assay. (F) RT-PCR analysis of hIL3 and mIL3 …

iPS cells generated without c-Myc have active Dlk1-Dio3 region and are capable of producing full-term mice through tetraploid complementation

W Li, X Zhao, H Wan, Y Zhang, L Liu, Z Lv, XJ Wang… - Cell research, 2011 - nature.com
… 3F-iPS cell lines to perform the tetraploid complementation assay. Viable full-term developed
… cells could produce viable mice through tetraploid complementation with the same rate as …

Rat embryonic stem cells produce fertile offspring through tetraploid complementation

TD Li, GH Feng, YF Li, M Wang… - Proceedings of the …, 2017 - National Acad Sciences
… produce live rats via tetraploid complementation and how the … can pass the tetraploid
complementation test for pluripotency. … for tetraploid complementation assay for rats was reliable. …

Mice generated from tetraploid complementation competent iPS cells show similar developmental features as those from ES cells but are prone to tumorigenesis

M Tong, Z Lv, L Liu, H Zhu, QY Zheng, XY Zhao, W Li… - Cell research, 2011 - nature.com
… capable of producing full-term mice via the tetraploid complementation method 3,4,5 , yet a
… of producing healthy mice through the tetraploid complementation assay (4N-iPSCs) 3 . The …