Previously, it was suggested that purple grain colour was transferred to bread wheat from purple-grained tetraploid T. durum . In the current study, we demonstrated that the D genome of bread wheat ‘Purple’ carries one of two complementary genes determining purple grain colour. This gene was mapped on the short arm of chromosome 7D 2.5 cM distal to the locus Rc-D1 determining red coleoptile colour. This position is highly comparable with that of the Pp1 gene mapped earlier on the short arm of chromosome 7B in tetraploid T. durum .We suggest the Pp genes on T. durum chromosome 7B and T. aestivum chromosome 7D are orthologous. We designated them Pp-B1 and Pp-D1 , respectively. Microsatellite-based genotyping of near-isogenic lines ‘i:S29Pp1Pp2 ^PF ’ and ‘i:S29Pp1Pp3 ^P ’, their recurrent ( T. aestivum ‘Saratovskaya 29’) and donor ( T. aestivum ‘Purple Feed’ and ‘Purple’, respectively) parents showed the presence of donor introgressions on chromosomes 2A and 7D in both near-isogenic lines. In addition to previously described purple pericarp, anthers and culms, phenotyping of these lines in the current study showed dark red coleoptile colour (with anthocyanin contents four times higher than in ‘Saratovskaya 29’ coleoptiles) and purple leaf blade and leaf sheath colour. It was concluded that each of the lines ‘i:S29Pp1Pp2 ^PF ’ and ‘i:S29Pp1Pp3 ^P ’ carry clusters of genes Rc-D1, Pc-D1, Pan-D1, Plb-D1, Pls-D1 and Pp-D1 on chromosome 7D between microsatellite markers Xgwm0044 and Xgwm0676 .