The goal of the study was to determine the influence of DSI test conditions, ie loading/unloading rates, hold time, the value of the maximum loading force on selected mechanical properties of trabecular bone tissue. The test samples were resected from a femoral head of a patient qualified for a hip replacement surgery. During the DSI tests hardness (HV, HM, HIT) and elastic modulus (EIT) of trabecular bone tissue were measured using the Micro Hardness Tester (MHT, CSEM). The analysis of the results of measurements and the calculations of total energy, ie elastic and inelastic (Wtotal, Welastic, Winelastic) and those of the parameters of hardness and elasticity made it possible to assess the impact of the process parameters (loading velocity, force and hold time) on mechanical properties of bone structures at a microscopic level. The coefficient k dependent on the ratio EIT/HIT and on the stored energy (ΔW= Wtotal-Welastic) is a measure of the material reaction to the loading and the deformation of tissue.