Saliva has attracted attention as a diagnostic fluid due to the association of oral microbiota
with systemic diseases. However, the lack of standardised methods for saliva collection has
led to the slow uptake of saliva in microbiome research. The aim of this study was to
systematically evaluate the potential effects on salivary microbiome profiles using different
methods of saliva collection, storage and gDNA extraction. Three types of saliva fractions
were collected from healthy individuals with or without the gDNA stabilising buffer …