The use of coded PCR primers enables high-throughput sequencing of multiple homolog amplification products by 454 parallel sequencing
Background The invention of the Genome Sequence 20™ DNA Sequencing System (454
parallel sequencing platform) has enabled the rapid and high-volume production of
sequence data. Until now, however, individual emulsion PCR (emPCR) reactions and
subsequent sequencing runs have been unable to combine template DNA from multiple
individuals, as homologous sequences cannot be subsequently assigned to their original
sources. Methodology We use conventional PCR with 5′-nucleotide tagged primers to …
parallel sequencing platform) has enabled the rapid and high-volume production of
sequence data. Until now, however, individual emulsion PCR (emPCR) reactions and
subsequent sequencing runs have been unable to combine template DNA from multiple
individuals, as homologous sequences cannot be subsequently assigned to their original
sources. Methodology We use conventional PCR with 5′-nucleotide tagged primers to …
[PDF][PDF] The Use of Coded PCR Primers Enables High-Throughput Sequencing of
J Binladen, MTP Gilbert, JP Bollback, F Panitz… - 2007 - pdfs.semanticscholar.org
Background. The invention of the Genome Sequence 20TM DNA Sequencing System (454
parallel sequencing platform) has enabled the rapid and high-volume production of
sequence data. Until now, however, individual emulsion PCR (emPCR) reactions and
subsequent sequencing runs have been unable to combine template DNA from multiple
individuals, as homologous sequences cannot be subsequently assigned to their original
sources. Methodology. We use conventional PCR with 5!-nucleotide tagged primers to …
parallel sequencing platform) has enabled the rapid and high-volume production of
sequence data. Until now, however, individual emulsion PCR (emPCR) reactions and
subsequent sequencing runs have been unable to combine template DNA from multiple
individuals, as homologous sequences cannot be subsequently assigned to their original
sources. Methodology. We use conventional PCR with 5!-nucleotide tagged primers to …
以上显示的是最相近的搜索结果。 查看全部搜索结果