Transcript profiling and lipidomic analysis of ceramide subspecies in mouse embryonic stem cells and embryoid bodies [S]
Ceramides (Cers) are important in embryogenesis, but no comprehensive analysis of gene
expression for Cer metabolism nor the Cer amounts and subspecies has been conducted
with an often used model: mouse embryonic stem cells (mESCs) versus embroid bodies
(EBs). Measuring the mRNA levels by quantitative RT-PCR and the amounts of the
respective metabolites by LC-ESI/MS/MS, notable differences between R1 mESCs and EBs
were: EBs have higher mRNAs for CerS1 and CerS3, which synthesize C18-and C≥ 24 …
expression for Cer metabolism nor the Cer amounts and subspecies has been conducted
with an often used model: mouse embryonic stem cells (mESCs) versus embroid bodies
(EBs). Measuring the mRNA levels by quantitative RT-PCR and the amounts of the
respective metabolites by LC-ESI/MS/MS, notable differences between R1 mESCs and EBs
were: EBs have higher mRNAs for CerS1 and CerS3, which synthesize C18-and C≥ 24 …
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