Transfection of Small Noncoding RNAs into Arabidopsis thaliana Protoplasts
S Lalande, M Chery, E Ubrig, G Hummel… - RNA Tagging: Methods …, 2020 - Springer
S Lalande, M Chery, E Ubrig, G Hummel, J Kubina, A Geldreich, L Drouard
RNA Tagging: Methods and Protocols, 2020•SpringerPolyethylene glycol transfection of plant protoplasts represents an efficient method to
incorporate foreign DNA and study transient gene expression. Here, we describe an
optimized protocol to deliver small noncoding RNAs into Arabidopsis thaliana protoplasts.
An example of application is provided by demonstrating the incorporation of a 20 nt long
small noncoding RNA deriving from the 5′ extremity of an A. thaliana cytosolic alanine
tRNA into freshly isolated protoplasts.
incorporate foreign DNA and study transient gene expression. Here, we describe an
optimized protocol to deliver small noncoding RNAs into Arabidopsis thaliana protoplasts.
An example of application is provided by demonstrating the incorporation of a 20 nt long
small noncoding RNA deriving from the 5′ extremity of an A. thaliana cytosolic alanine
tRNA into freshly isolated protoplasts.
Abstract
Polyethylene glycol transfection of plant protoplasts represents an efficient method to incorporate foreign DNA and study transient gene expression. Here, we describe an optimized protocol to deliver small noncoding RNAs into Arabidopsis thaliana protoplasts. An example of application is provided by demonstrating the incorporation of a 20 nt long small noncoding RNA deriving from the 5′ extremity of an A. thaliana cytosolic alanine tRNA into freshly isolated protoplasts.
Springer
以上显示的是最相近的搜索结果。 查看全部搜索结果