Transgenic quail production by microinjection of lentiviral vector into the early embryo blood vessels
Z Zhang, P Sun, F Yu, L Yan, F Yuan, W Zhang… - PLoS …, 2012 - journals.plos.org
Several strategies have been used to generate transgenic birds. The most successful
method so far has been the injection of lentiviral vectors into the subgerminal cavity of a
newly laid egg. We report here a new, easy and effective way to produce transgenic quails
through direct injection of a lentiviral vector, containing an enhanced-green fluorescent
protein (eGFP) transgene, into the blood vessels of quail embryos at Hamburger-Hamilton
stage 13–15 (HH13–15). A total of 80 embryos were injected and 48 G0 chimeras (60%) …
method so far has been the injection of lentiviral vectors into the subgerminal cavity of a
newly laid egg. We report here a new, easy and effective way to produce transgenic quails
through direct injection of a lentiviral vector, containing an enhanced-green fluorescent
protein (eGFP) transgene, into the blood vessels of quail embryos at Hamburger-Hamilton
stage 13–15 (HH13–15). A total of 80 embryos were injected and 48 G0 chimeras (60%) …
Transgenic quail production by microinjection of lentiviral vector into the early embryo blood vessels.
ZZF Zhang ZiFu, SP Sun Peng, YFX Yu FuXian… - 2012 - cabidigitallibrary.org
Several strategies have been used to generate transgenic birds. The most successful
method so far has been the injection of lentiviral vectors into the subgerminal cavity of a
newly laid egg. We report here a new, easy and effective way to produce transgenic quails
through direct injection of a lentiviral vector, containing an enhanced-green fluorescent
protein (eGFP) transgene, into the blood vessels of quail embryos at Hamburger-Hamilton
stage 13-15 (HH13-15). A total of 80 embryos were injected and 48 G0 chimeras (60%) …
method so far has been the injection of lentiviral vectors into the subgerminal cavity of a
newly laid egg. We report here a new, easy and effective way to produce transgenic quails
through direct injection of a lentiviral vector, containing an enhanced-green fluorescent
protein (eGFP) transgene, into the blood vessels of quail embryos at Hamburger-Hamilton
stage 13-15 (HH13-15). A total of 80 embryos were injected and 48 G0 chimeras (60%) …
以上显示的是最相近的搜索结果。 查看全部搜索结果