To determine if a treadmill-workstation (TMWS) increases physical activity (PA) and influences anthropometric, body composition, cardiovascular, and metabolic variables in overweight and obese office-workers.

Twelve (mean age= 46.2 ± 9.2 years) overweight/obese sedentary office-workers (mean BMI= 33.9 ± 5.0 kg·m ^-2 ) volunteered to participate in this 9-month study. After baseline measurements of postural allocation, steps per day, anthropometric variables, body composition, cardiovascular, and metabolic variables, TMWS were installed in the participants’ offices for their use. Baseline measurements were repeated after 3 and 9 months. Comparisons of the outcome variables were made using repeated-measures ANOVAs or nonparametric Friedman’s Rank Tests.

Between baseline and 9 months, significant increases were seen in the median standing (146−203 min·day ^-1 ) and stepping time (52−90 min·day ^-1 ) and total steps/day (4351−7080 steps/day; P < .05). Correspondingly, the median time spent sitting/lying decreased (1238−1150 min·day ^-1 ; P < .05). Using the TMWS significantly reduced waist (by 5.5 cm) and hip circumference (by 4.8 cm), low-density lipoproteins (LDL) (by 16 mg·dL ^-1 ), and total cholesterol (by 15 mg·dL ^-1 ) during the study ( P < .05).

The additional PA energy expenditure from using the TMWS favorably influenced waist and hip circumferences and lipid and metabolic profiles in overweight and obese office-workers.