With the ever-increasing advancements in the technology of driver assistant systems, there is a need for a comprehensive way to identify traffic conflicts to avoid collisions. Although significant research efforts have been devoted to traffic conflict techniques applied for junctions, there is dearth of research on these methods for motorways. This paper presents the validation of a traffic conflict prediction algorithm applied to a motorway scenario in a simulated environment. An automatic video analysis system was developed to identify lane change and rear-end conflicts as ground truth. Using these conflicts, the prediction ability of the traffic conflict technique was validated in an integrated simulation framework. This framework consisted of a sub-microscopic simulator, which provided an appropriate testbed to accurately simulate the components of an intelligent vehicle, and a microscopic traffic simulator able to generate the surrounding traffic. Results from this framework show that for a 10% false alarm rate, approximately 80% and 73% of rear-end and lane change conflicts were accurately predicted, respectively. Despite the fact that the algorithm was not trained using the virtual data, the sensitivity was high. This highlights the transferability of the algorithm to similar road networks, providing a benchmark for the identification of traffic conflict and a relevant step for developing safety management strategies for autonomous vehicles.