In some lysosomal storage diseases (LSD) cholesterol accumulates in vesicles. Whether
increased vesicle cholesterol affects vesicle fusion with the plasmalemma, where the fusion
pore, a channel between the vesicle lumen and the extracellular space, is formed, is
unknown. Super-resolution microscopy revealed that after stimulation of exocytosis, pituitary
lactotroph vesicles discharge cholesterol which transfers to the plasmalemma. Cholesterol
depletion in lactotrophs and astrocytes, both exhibiting Ca 2+-dependent exocytosis …