Thermodynamic analysis of protein interactions with biosensor technology

H Roos, R Karlsson, H Nilshans… - Journal of Molecular …, 1998 - Wiley Online Library
… The highest level of thermodynamic analysis in this investigation is to use the kinetic data
and apply transition state analysis. Table 3 and Figs 5,6,7 show the results of this …

Thermodynamic analysis of antigen–antibody binding using biosensor measurements at different temperatures

G Zeder-Lutz, E Zuber, J Witz… - Analytical …, 1997 - Elsevier
… However, in all these studies, DHcal has been calculated from calorimetric data, ie, with the
biosensor technology, compared to the steady decrease of ln K with temperature reported by …

Potential of biosensor technology for the characterization of interactions by quantitative affinity chromatography

DR Hall, DJ Winzor - Journal of Chromatography B: Biomedical Sciences …, 1998 - Elsevier
biosensor technology in the broader context of their study by quantitative affinity chromatography.
The general reluctance to consider biosensor… as thermodynamic analyses of biosensor

Kinetic, equilibrium, and thermodynamic analysis of macromolecular interactions with BIACORE

DG Myszka - Methods in enzymology, 2000 - Elsevier
… extracting thermodynamic information from biosensor analysis requires van't Hoff analysis
to determine the enthalpy change. While biosensors can quantitate the amount of complex …

[HTML][HTML] Real-time and label-free analysis of binding thermodynamics of carbohydrate-protein interactions on unfixed cancer cell surfaces using a QCM biosensor

X Li, S Song, Q Shuai, Y Pei, T Aastrup, Y Pei, Z Pei - Scientific reports, 2015 - nature.com
… surface carbohydrates was used as a model system for the thermodynamic analysis of
biomolecule interaction occurring on cell surface by QCM biosensor. The affinity constant (K D ) of …

A thermodynamic characterization of the binding of thrombin inhibitors to human thrombin, combining biosensor technology, stopped-flow spectrophotometry, and …

J Deinum, L Gustavsson, E Gyzander… - Analytical …, 2002 - Elsevier
… of immobilized thrombin were used in the thermodynamic analyses of the highaffinity binders.
Surfaces with more than 4 kRU were used for KD analysis after further stabilization by an …

Biphasic thermodynamic characterization of interactions by quantitative affinity chromatography and biosensor technology

DJ Winzor - Journal of Chromatography A, 1998 - Elsevier
… by kinetic analysis of biosensor traces. Because … thermodynamic analysis (A+X⇄AX), the
parameters thus determined are necessarily the rate constants pertaining to the thermodynamic

Thermodynamic benchmark study using Biacore technology

I Navratilova, GA Papalia, RL Rich, D Bedinger… - Analytical …, 2007 - Elsevier
… that the affinities and thermodynamics determined using the biosensor matched the values
… consumption, thermodynamic analysis of interactions using Biacore biosensors can be done …

Direct comparison of binding equilibrium, thermodynamic, and rate constants determined by surface‐and solution‐based biophysical methods

YSN Day, CL Baird, RL Rich, DG Myszka - Protein Science, 2002 - Wiley Online Library
… resonance biosensor experiments are performed with care, the equilibrium, thermodynamic,
and … These results validate the use of biosensor technology to collect reliable data on small …

Thermodynamic analysis of the interaction between cationic porphyrins and human serum albumin by an optical biosensor

Q Yi‐Min, P Ni‐Na, J Yong‐Xing… - Chinese Journal of …, 2005 - Wiley Online Library
… of material required for typical analyses (generally requiring about 1 µg … the technology to
kinetic and thermodynamic analysis of … HSA using an optical biosensor with those obtained by …