Concentration measurement of unpurified proteins using biosensor technology under conditions of partial mass transport limitation

PM Richalet-Sécordel, N Rauffer-Bruyère… - Analytical …, 1997 - Elsevier
concentration measured by OD was twice that obtained dextran matrix does not affect the
binding under conditions suitable for kinetic measurements… our concentration measurements is …

… and dissociation rate constants of interacting biomolecules: an analytical solution to the theory for kinetic and mass transport limitations in biosensor technology and …

K Sigmundsson, G Másson, R Rice, N Beauchemin… - Biochemistry, 2002 - ACS Publications
… (SPR) detection of the specific binding of GST−Lcyt-YF to the anti-phosphotyrosine
antibody, and compared with the active concentration measured by radioactivity determination. …

Limitation of the transport capacity approach in sediment transport modeling

GC Sander, JY Parlange, DA Barry… - Water Resources …, 2007 - Wiley Online Library
… Sediment concentrations were then measured at different … and the suspended sediment
concentration are different for both … the transport capacity for describing sediment transport under …

Mass-transport limitations in spot-based microarrays

M Zhao, X Wang, D Nolte - Biomedical optics express, 2010 - opg.optica.org
… the low concentration limit, the shapes of the mass-transport-… that it is more sensitive to
measure the spatial binding … the mass-transport-limited regime and measuring the binding …

Investigating fuel-cell transport limitations using hydrogen limiting current

FB Spingler, A Phillips, T Schuler, MC Tucker… - International Journal of …, 2017 - Elsevier
… argon, the average hydrogen concentration in the flow field … hydrogen concentration at the
reaction sites is zero (ie limiting … study was to perform limiting current measurements with up to …

Absolute quantification of microRNAs based on mass transport limitation under a laminar flow SPR system

K Li, N An, L Wu, M Wang, F Li, L Li - Biosensors and Bioelectronics, 2024 - Elsevier
… of the analyte and its absolute concentration to achieve accurate measurements. The key
to this approach is the measurement of the mass transport rate of the analyte molecules from …

The role of mass transport limitation and surface heterogeneity in the biophysical characterization of macromolecular binding processes by SPR biosensing

P Schuck, H Zhao - Surface plasmon resonance: methods and protocols, 2010 - Springer
transport limitation (see below). In order to establish that the data are single exponential and
to enable us measuring … mass transport limitation where the spatial concentration gradients …

Effect of solute concentration and mass transfer limitations on transport in organic solvent nanofiltration—partially rejected solute

P Silva, AG Livingston - Journal of membrane science, 2006 - Elsevier
… , making it difficult to distinguish between the concentration effect and the experimental error
associated with the measurements. This is not surprising, given the quoted MWCO of 700 g …

Solute transport in hemodialysis: advances and limitations of current membrane technology

WR Clark, D Gao, M Neri, C Ronco - Expanded Hemodialysis, 2017 - karger.com
measured blood concentration. However, when secondary membrane and polarization
effects are prominent (eg, at high ultrafiltration rates), the relationship between the sieving and …

Kinetics of antigen binding to antibody microspots: strong limitation by mass transport to the surface

W Kusnezow, YV Syagailo, S Rüffer, K Klenin… - …, 2006 - Wiley Online Library
… 5A that the progression curve for 256Kd concentration runs almost linearly instead of …
reactions measured, eg. by Biacore systems may suffer also from mass-transport limitations in spite …