Fluorescence microscopy beyond the diffraction limit

M Heilemann - Journal of biotechnology, 2010 - Elsevier
… From the successful experimental realization of fluorescence microscopy below the diffraction
limit and its first meaningful applications, we can anticipate an important contribution to …

Far-field fluorescence microscopy beyond the diffraction limit

AM van Oijen, J Köhler, J Schmidt, M Müller… - JOSA A, 1999 - opg.optica.org
… This study demonstrates that one can circumvent the diffraction limit, which dictates the
resolution in far-field confocal microscopy, by combining position-sensitive imaging with spectral …

Shattering the diffraction limit of light: a revolution in fluorescence microscopy?

S Weiss - Proceedings of the National Academy of …, 2000 - National Acad Sciences
microscopy, and in particular fluorescence microscopy, will play a vital role in this endeavor.
Fluorescence microscopy … The developments of green (and other color) fluorescent proteins …

Beyond the diffraction limit: far-field fluorescence imaging with ultrahigh resolution

JH Rice - Molecular BioSystems, 2007 - pubs.rsc.org
… Demand for improved resolution has stimulated research into developing methods to image
beyond the diffraction limit based on far-field fluorescence microscopy techniques. Rapid …

Overcoming diffraction limit: From microscopy to nanoscopy

S So, M Kim, D Lee, DM Nguyen… - Applied Spectroscopy …, 2018 - Taylor & Francis
… the diffraction limit of fluorescence microscopy, numerous techniques such as super-resolution
microscopy … optical microscopic techniques from fluorescence microscopy to label-free …

Breaking the Axial Diffraction Limit: A Guide to Axial Super‐Resolution Fluorescence Microscopy

W Liu, KC Toussaint Jr, C Okoro, D Zhu… - Laser & Photonics …, 2018 - Wiley Online Library
… its resolving ability remained limited by the optical diffraction limit. Super-resolution
fluorescence microscopy (SRFM) has been introduced to break the diffraction limit and significantly …

Breaking the diffraction limit of light-sheet fluorescence microscopy by RESOLFT

P Hoyer, G De Medeiros, B Balázs… - Proceedings of the …, 2016 - National Acad Sciences
… -beam LS, exploiting the dark and fluorescent states of the RSFPs to reduce the axial extent
of the examined object layer below the diffraction limit. This enables subdiffraction optical …

Breaking the diffraction barrier in fluorescence microscopy by optical shelving

S Bretschneider, C Eggeling, SW Hell - Physical review letters, 2007 - APS
… of the diffraction resolution barrier in far-field fluorescence microscopy by transiently … featuring
a local zero, we confine the fluorescence emission to a spot whose diameter is a fraction of …

Breaking Abbe's diffraction resolution limit in fluorescence microscopy with stimulated emission depletion beams of various shapes

TA Klar, E Engel, SW Hell - Physical Review E, 2001 - APS
… to break the diffraction barrier in fluorescence microscopy by … The farfield microscopy
diffraction limit largely remained an … the diffraction resolution limit in far-field fluorescence micros…

Ground-state-depletion fluorscence microscopy: A concept for breaking the diffraction resolution limit

SW Hell, M Kroug - Applied Physics B, 1995 - Springer
fluorescence microscopy fundamentally overcoming the classical diffraction resolution limit.
… of the effective focus of a scanning fluorescence microscope. The reduction is achieved by …