metabaR: An r package for the evaluation and improvement of DNA metabarcoding data quality
L Zinger, C Lionnet, AS Benoiston… - Methods in Ecology …, 2021 - Wiley Online Library
DNA metabarcoding is becoming the tool of choice for biodiversity assessment across taxa
and environments. Yet, the artefacts present in metabarcoding datasets often preclude a
proper interpretation of ecological patterns. Bioinformatic pipelines to remove experimental
noise exist. However, these often only partially target produced artefacts, or are marker
specific. In addition, assessments of data curation quality and chosen filtering thresholds are
seldom available in existing pipelines, partly due to the lack of appropriate visualisation …
and environments. Yet, the artefacts present in metabarcoding datasets often preclude a
proper interpretation of ecological patterns. Bioinformatic pipelines to remove experimental
noise exist. However, these often only partially target produced artefacts, or are marker
specific. In addition, assessments of data curation quality and chosen filtering thresholds are
seldom available in existing pipelines, partly due to the lack of appropriate visualisation …
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