Comparison of methyl-DNA immunoprecipitation (MeDIP) and methyl-CpG binding domain (MBD) protein capture for genome-wide DNA methylation analysis reveal …
… enrich for methylated DNA regions of the genome, namely methylDNA immunoprecipitation
(… (5MeC) and capture of methylated DNA using a methyl-CpG binding domain-based (MBD) …
(… (5MeC) and capture of methylated DNA using a methyl-CpG binding domain-based (MBD) …
Methyl-DNA immunoprecipitation (MeDIP): hunting down the DNA methylome
FV Jacinto, E Ballestar, M Esteller - Biotechniques, 2008 - Taylor & Francis
… , several strategies involving the specific interaction of proteins with methylated DNA have …
family of proteins, which are chosen for their selectivity in binding methylated DNA. Strategies …
family of proteins, which are chosen for their selectivity in binding methylated DNA. Strategies …
Methyl DNA immunoprecipitation
JJ Goval, J Magdalena - … Immunoprecipitation Assays: Methods and …, 2009 - Springer
… the protein association with specific genomic regions in the context of intact cells. ChIP and
immunoprecipitation (IP) of methylated DNA… results due to capture of unmethylated DNA. The …
immunoprecipitation (IP) of methylated DNA… results due to capture of unmethylated DNA. The …
[HTML][HTML] Optimized method for methylated DNA immuno-precipitation
C Guerrero-Bosagna, P Jensen - MethodsX, 2015 - Elsevier
… Methylated DNA immunoprecipitation (MeDIP) is one of the … of the captured methylated DNA
in order to detect local DNA … after the digestion reaction with protein K. After a wide range of …
in order to detect local DNA … after the digestion reaction with protein K. After a wide range of …
Enrichment of methylated DNA by methyl-CpG immunoprecipitation
M Sonnet, C Baer, M Rehli, D Weichenhan… - Lymphoma: Methods and …, 2013 - Springer
… fusion protein, … protein is immobilized to protein A-coated sepharose or magnetic bead
particles. Upon incubation with fragmented sample DNA, MBD2 selectively captures methylated …
particles. Upon incubation with fragmented sample DNA, MBD2 selectively captures methylated …
Methylation analysis by DNA immunoprecipitation
KL Thu, LA Pikor, JY Kennett… - Journal of cellular …, 2010 - Wiley Online Library
… , anti-5mC, or a recombinant methyl binding protein. A secondary antibody captures the
methylated DNA–antibody/protein complex. The immunoprecipitated DNA can then be analyzed …
methylated DNA–antibody/protein complex. The immunoprecipitated DNA can then be analyzed …
Methyl-cytosine-based immunoprecipitation for DNA methylation analysis
PM Lizardi, Q Yan… - Cold Spring Harbor …, 2017 - cshprotocols.cshlp.org
… higher affinity for methylated DNA than MBD2b alone RiboMed (MethylMagnet) … proteins
and glutathione magnetic beads to capture methylated DNA Can be used to isolate mCpG DNA …
and glutathione magnetic beads to capture methylated DNA Can be used to isolate mCpG DNA …
Microplate-based platform for combined chromatin and DNA methylation immunoprecipitation assays
J Yu, Q Feng, Y Ruan, R Komers, N Kiviat… - BMC Molecular …, 2011 - Springer
… digestion, and methyl-DNA immunoprecipitation (MeDIP) are … sealing film and capture of
chromatin and DNA complexes to … To do that we used 96-well protein A-coated polypropylene …
chromatin and DNA complexes to … To do that we used 96-well protein A-coated polypropylene …
Methylation analysis by DNA immunoprecipitation (MeDIP)
… , and incubated with primary antibody specific to methylated cytosine (3). A … methylated DNA
is thereby captured by use of a magnetic rack. IP reaction is then purified to remove proteins …
is thereby captured by use of a magnetic rack. IP reaction is then purified to remove proteins …
Detection of DNA methylation by MeDIP and MBDCap assays: an overview of techniques
HK Hsu, YI Weng, PY Hsu, THM Huang… - Molecular Toxicology …, 2014 - Springer
… methylation hybridization (DMH), methyl-DNA immunoprecipitation (MeDIP) combined with
… , MBDCap captures double stranded methylated DNA fragments by MBD-based proteins and …
… , MBDCap captures double stranded methylated DNA fragments by MBD-based proteins and …
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