… quantitative PCR methods, real-time PCR does not require post-PCR sample handling,
preventing potential PCR … The real-time PCR method has a very large dynamic range of starting …

… Real-time quantitative PCR allows the sensitive, specific and reproducible quantitation of
nucleic acids. Since its introduction, real-time quantitative PCR … lower turnaround times. This …

… Standard techniques for counting parasites are often time-consuming, difficult and
inaccurate, and occasionally unpleasant. Real-time quantitative polymerase chain reaction has …

… Real-time quantitative PCR is the reliable detection and measurement of products generated
during each cycle of the polymerase chain reaction (PCR… to the start of the PCR process. To …

… RT-PCR enables quantification of the line (22, 23). Thus, this value expresses the probe
degraPCR product in “real-time.” This sensitive and accuratedation during the PCR process. …

… with conventional quantitative PCR, thus allowing the routine and reliable quantitation of PCR
… Two fluorogenic probes are available for use on real time quantitation. The fluorogenic 5'-…

… Real-time PCR was performed on the nal control genes. Suitable internal controls for realtime
quantitative PCR include GAPDH, β-actin, β2- corresponding cDNA synthesized from …

… Real-time quantitative PCR combines PCR and fluorescent reporter chemistry to monitor
template amplification with higher sensitivity and specificity. Quantitative PCR-… quantitative PCR …

… Real-time quantitative PCR. Real-time quantitative PCR was performed on an MJ Opticon
system (MJ Research), using SYBR green JumpStart Taq ReadyMix regents (Sigma) in a 20 …

… than quantitative PCR, which limits the interpretation of results and slows down sample
screening. We present real-time qPCR … The only requirement is for the real-time machine to be …